Whitby Lab

Rad52’s DNA annealing activity drives template switching associated with restarted replication. Kishkevich A, Tamang S, Nguyen M, Oehler J, Bulmaga E, Andreadis C, Morrow CA, Osman F and Whitby MC. Nat. Commun. 2022 Nov 26; 13, 7293 https://doi.org/10.1038/s41467-022-35060-4.

The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast. Wong IN, Neo JPS, Oehler J, Schafhauser S, Osman F, Carr SB, Whitby MC. Elife. 2019 Dec 19;8. pii: e49784. doi: 10.7554/eLife.49784.

DNA sequence differences are determinants of meiotic recombination outcome. Brown SD, Mpaulo SJ, Asogwa MN, Jézéquel M, Whitby MC, Lorenz A. Sci Rep. 2019 Nov 11;9(1):16446. doi: 10.1038/s41598-019-52907-x.

The PCNA unloader Elg1 promotes recombination at collapsed replication forks in fission yeast. Tamang S, Kishkevich A, Morrow CA, Osman F, Jalan M and Whitby MC. Elife. 2019 May 31;8. pii: e47277. doi: 10.7554/eLife.47277.

Factors affecting template switch recombination associated with restarted DNA replication. Jalan M, Oehler J, Morrow CA, Osman F and Whitby MC. Elife. 2019 Jan 22;8. pii: e41697. doi: 10.7554/eLife.41697.

Inter-Fork Strand Annealing causes genomic deletions during the termination of DNA replication. Morrow CA, Nguyen MO, Fower A, Wong IN, Osman F, Bryer C, Whitby MC. Elife. 2017 Jun 6;6. pii: e25490. doi: 10.7554/eLife.25490.

The RecQ DNA helicase Rqh1 constrains Exonuclease 1-dependent recombination at stalled replication forks. Osman F, Ahn JS, Lorenz A, Whitby MC. Sci Rep. 2016 Mar 9;6:22837. doi: 10.1038/srep22837.

Recombination occurs within minutes of replication blockage by RTS1 producing restarted forks that are prone to collapse. Nguyen MO, Jalan M, Morrow CA, Osman F, Whitby MC. Elife. 2015 Mar 25;4:e04539. doi: 10.7554/eLife.04539.

Sumoylation influences DNA break repair partly by increasing the solubility of a conserved end resection protein. Sarangi P, Steinacher R, Altmannova V, Fu Q, Paull TT, Krejci L, Whitby MC, Zhao X. PLoS Genet. 2015 Jan 8;11(1):e1004899. doi: 10.1371/journal.pgen.1004899. eCollection 2015 Jan.

Rad51/Dmc1 paralogs and mediators oppose DNA helicases to limit hybrid DNA formation and promote crossovers during meiotic recombination. Lorenz A, Mehats A, Osman F, Whitby MC. Nucleic Acids Res. 2014 Dec 16;42(22):13723-35. doi: 10.1093/nar/gku1219. Epub 2014 Nov 20.

Emerging roles for centromere-associated proteins in DNA repair and genetic recombination. Osman F, Whitby MC. Biochem Soc Trans. 2013 Dec;41(6):1726-30. doi: 10.1042/BST20130200. Review.

MHF1-2/CENP-S-X performs distinct roles in centromere metabolism and genetic recombination. Bhattacharjee S, Osman F, Feeney L, Lorenz A, Bryer C, Whitby MC. Open Biol. 2013 Sep 11;3(9):130102. doi: 10.1098/rsob.130102.

Slx8 removes Pli1-dependent protein-SUMO conjugates including SUMOylated topoisomerase I to promote genome stability. Steinacher R, Osman F, Lorenz A, Bryer C, Whitby MC. PLoS One. 2013 Aug 6;8(8):e71960. doi: 10.1371/journal.pone.0071960. Print 2013.

Structure-specific endonucleases xpf and mus81 play overlapping but essential roles in DNA repair by homologous recombination. Kikuchi K, Narita T, Pham VT, Iijima J, Hirota K, Keka IS, Mohiuddin, Okawa K, Hori T, Fukagawa T, Essers J, Kanaar R, Whitby MC, Sugasawa K, Taniguchi Y, Kitagawa K, Takeda S. Cancer Res. 2013 Jul 15;73(14):4362-71. doi: 10.1158/0008-5472.CAN-12-3154. Epub 2013 Apr 10.

How not to get cross(ed): a novel role for FANCM orthologs in meiotic recombination. Lorenz A, Whitby MC. Cell Cycle. 2012 Sep 15;11(18):3347-8. doi: 10.4161/cc.21844. Epub 2012 Aug 23.

The ATPase activity of Fml1 is essential for its roles in homologous recombination and DNA repair. Nandi S, Whitby MC. Nucleic Acids Res. 2012 Oct;40(19):9584-95. doi: 10.1093/nar/gks715. Epub 2012 Jul 27.

The fission yeast FANCM ortholog directs non-crossover recombination during meiosis. Lorenz A, Osman F, Sun W, Nandi S, Steinacher R, Whitby MC. Science. 2012 Jun 22;336(6088):1585-8. doi: 10.1126/science.1220111.

The DNA helicase Pfh1 promotes fork merging at replication termination sites to ensure genome stability. Steinacher R, Osman F, Dalgaard JZ, Lorenz A, Whitby MC. Genes Dev. 2012 Mar 15;26(6):594-602. doi: 10.1101/gad.184663.111.

Ultrafine anaphase bridges, broken DNA and illegitimate recombination induced by a replication fork barrier. Sofueva S, Osman F, Lorenz A, Steinacher R, Castagnetti S, Ledesma J, Whitby MC. Nucleic Acids Res. 2011 Aug;39(15):6568-84. doi: 10.1093/nar/gkr340. Epub 2011 May 16.

A failure of meiotic chromosome segregation in a fbh1Delta mutant correlates with persistent Rad51-DNA associations. Sun W, Lorenz A, Osman F, Whitby MC. Nucleic Acids Res. 2011 Mar;39(5):1718-31. doi: 10.1093/nar/gkq977. Epub 2010 Dec 11.

A histone-fold complex and FANCM form a conserved DNA-remodeling complex to maintain genome stability. Yan Z, Delannoy M, Ling C, Daee D, Osman F, Muniandy PA, Shen X, Oostra AB, Du H, Steltenpool J, Lin T, Schuster B, Décaillet C, Stasiak A, Stasiak AZ, Stone S, Hoatlin ME, Schindler D, Woodcock CL, Joenje H, Sen R, de Winter JP, Li L, Seidman MM, Whitby MC, Myung K, Constantinou A, Wang W. Mol Cell. 2010 Mar 26;37(6):865-78. doi: 10.1016/j.molcel.2010.01.039.

The FANCM family of DNA helicases/translocases. Whitby MC. DNA Repair (Amst). 2010 Mar 2;9(3):224-36. doi: 10.1016/j.dnarep.2009.12.012. Epub 2010 Feb 8. Review.

The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant. Lorenz A, West SC, Whitby MC. Nucleic Acids Res. 2010 Apr;38(6):1866-73. doi: 10.1093/nar/gkp1179. Epub 2009 Dec 29.

Monitoring homologous recombination following replication fork perturbation in the fission yeast Schizosaccharomyces pombe. Osman F, Whitby MC. Methods Mol Biol. 2009;521:535-52. doi: 10.1007/978-1-60327-815-7_31.

Fbh1 limits Rad51-dependent recombination at blocked replication forks. Lorenz A, Osman F, Folkyte V, Sofueva S, Whitby MC. Mol Cell Biol. 2009 Sep;29(17):4742-56. doi: 10.1128/MCB.00471-09. Epub 2009 Jun 22.

Efficient second strand cleavage during Holliday junction resolution by RuvC requires both increased junction flexibility and an exposed 5′ phosphate. Osman F, Gaskell L, Whitby MC. PLoS One. 2009;4(4):e5347. doi: 10.1371/journal.pone.0005347. Epub 2009 Apr 28.

Alternative induction of meiotic recombination from single-base lesions of DNA deaminases. Pauklin S, Burkert JS, Martin J, Osman F, Weller S, Boulton SJ, Whitby MC, Petersen-Mahrt SK. Genetics. 2009 May;182(1):41-54. doi: 10.1534/genetics.109.101683. Epub 2009 Feb 23.

The FANCM ortholog Fml1 promotes recombination at stalled replication forks and limits crossing over during DNA double-strand break repair. Sun W, Nandi S, Osman F, Ahn JS, Jakovleska J, Lorenz A, Whitby MC. Mol Cell. 2008 Oct 10;32(1):118-28. doi: 10.1016/j.molcel.2008.08.024.

Exploring the roles of Mus81-Eme1/Mms4 at perturbed replication forks. Osman F, Whitby MC. DNA Repair (Amst). 2007 Jul 1;6(7):1004-17. Epub 2007 Apr 3. Review.

Correction of proliferation and drug sensitivity defects in the progeroid Werner’s Syndrome by Holliday junction resolution. Rodriguez-Lopez AM, Whitby MC, Borer CM, Bachler MA, Cox LS. Rejuvenation Res. 2007 Mar;10(1):27-40.

Mus81 cleavage of Holliday junctions: a failsafe for processing meiotic recombination intermediates? Gaskell LJ, Osman F, Gilbert RJ, Whitby MC. EMBO J. 2007 Apr 4;26(7):1891-901. Epub 2007 Mar 15.

Crossover promotion and prevention. Lorenz A, Whitby MC. Biochem Soc Trans. 2006 Aug;34(Pt 4):537-41. Review.

Making crossovers during meiosis. Whitby MC. Biochem Soc Trans. 2005 Dec;33(Pt 6):1451-5. Review.

The F-Box DNA helicase Fbh1 prevents Rhp51-dependent recombination without mediator proteins. Osman F, Dixon J, Barr AR, Whitby MC. Mol Cell Biol. 2005 Sep;25(18):8084-96.

Replication fork blockage by RTS1 at an ectopic site promotes recombination in fission yeast. Ahn JS, Osman F, Whitby MC. EMBO J. 2005 Jun 1;24(11):2011-23. Epub 2005 May 5.

Biochemical characterization and DNA repair pathway interactions of Mag1-mediated base excision repair in Schizosaccharomyces pombe. Alseth I, Osman F, Korvald H, Tsaneva I, Whitby MC, Seeberg E, Bjørås M. Nucleic Acids Res. 2005 Feb 18;33(3):1123-31. Print 2005.

DNA repair by a Rad22-Mus81-dependent pathway that is independent of Rhp51. Doe CL, Osman F, Dixon J, Whitby MC. Nucleic Acids Res. 2004 Oct 14;32(18):5570-81. Print 2004.

Junctions on the road to cancer. Whitby MC. Nat Struct Mol Biol. 2004 Aug;11(8):693-5.

The involvement of Srs2 in post-replication repair and homologous recombination in fission yeast. Doe CL, Whitby MC. Nucleic Acids Res. 2004 Mar 1;32(4):1480-91. Print 2004.

Generating crossovers by resolution of nicked Holliday junctions: a role for Mus81-Eme1 in meiosis. Osman F, Dixon J, Doe CL, Whitby MC. Mol Cell. 2003 Sep;12(3):761-74.

The role of the SAP motif in promoting Holliday junction binding and resolution by SpCCE1. Ahn JS, Whitby MC. J Biol Chem. 2003 Aug 1;278(31):29121-9. Epub 2003 May 14.

Cleavage of model replication forks by fission yeast Mus81-Eme1 and budding yeast Mus81-Mms4. Whitby MC, Osman F, Dixon J. J Biol Chem. 2003 Feb 28;278(9):6928-35. Epub 2002 Dec 8.

Mus81-Eme1 and Rqh1 involvement in processing stalled and collapsed replication forks. Doe CL, Ahn JS, Dixon J, Whitby MC. J Biol Chem. 2002 Sep 6;277(36):32753-9. Epub 2002 Jun 25.

UV irradiation causes the loss of viable mitotic recombinants in Schizosaccharomyces pombe cells lacking the G(2)/M DNA damage checkpoint. Osman F, Tsaneva IR, Whitby MC, Doe CL. Genetics. 2002 Mar;160(3):891-908.

Fission yeast nascent polypeptide-associated complex binds to four-way DNA junctions. Whitby MC, Dixon J. J Mol Biol. 2001 Mar 2;306(4):703-16.

The Holliday junction resolvase SpCCE1 prevents mitochondrial DNA aggregation in Schizosaccharomyces pombe. Doe CL, Osman F, Dixon J, Whitby MC. Mol Gen Genet. 2000 Jul;263(6):889-97.

Partial suppression of the fission yeast rqh1(-) phenotype by expression of a bacterial Holliday junction resolvase. Doe CL, Dixon J, Osman F, Whitby MC. EMBO J. 2000 Jun 1;19(11):2751-62.

Substrate specificity of the SpCCE1 holliday junction resolvase of Schizosaccharomyces pombe. Whitby MC, Dixon J. J Biol Chem. 1998 Dec 25;273(52):35063-73.

Targeting Holliday junctions by the RecG branch migration protein of Escherichia coli. Whitby MC, Lloyd RG. J Biol Chem. 1998 Jul 31;273(31):19729-39.

A new Holliday junction resolving enzyme from Schizosaccharomyces pombe that is homologous to CCE1 from Saccharomyces cerevisiae. Whitby MC, Dixon J. J Mol Biol. 1997 Oct 3;272(4):509-22.

Sequence specificity and biochemical characterization of the RusA Holliday junction resolvase of Escherichia coli. Chan SN, Harris L, Bolt EL, Whitby MC, Lloyd RG. J Biol Chem. 1997 Jun 6;272(23):14873-82.

Interactions between RuvA and RuvC at Holliday junctions: inhibition of junction cleavage and formation of a RuvA-RuvC-DNA complex. Whitby MC, Bolt EL, Chan SN, Lloyd RG. J Mol Biol. 1996 Dec 20;264(5):878-90.

Branch migration of three-strand recombination intermediates by RecG, a possible pathway for securing exchanges initiated by 3′-tailed duplex DNA. Whitby MC, Lloyd RG. EMBO J. 1995 Jul 17;14(14):3302-10.

Altered SOS induction associated with mutations in recF, recO and recR. Whitby MC, Lloyd RG. Mol Gen Genet. 1995 Jan 20;246(2):174-9.

Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions. Sharples GJ, Chan SN, Mahdi AA, Whitby MC, Lloyd RG. EMBO J. 1994 Dec 15;13(24):6133-42.

Branch migration of Holliday junctions: identification of RecG protein as a junction specific DNA helicase. Whitby MC, Vincent SD, Lloyd RG. EMBO J. 1994 Nov 1;13(21):5220-8.

Mutation of recF, recJ, recO, recQ, or recR improves Hfr recombination in resolvase-deficient ruv recG strains of Escherichia coli. Ryder L, Whitby MC, Lloyd RG. J Bacteriol. 1994 Mar;176(6):1570-7.

A mutation in helicase motif III of E. coli RecG protein abolishes branch migration of Holliday junctions. Sharples GJ, Whitby MC, Ryder L, Lloyd RG. Nucleic Acids Res. 1994 Feb 11;22(3):308-13.

Reverse branch migration of Holliday junctions by RecG protein: a new mechanism for resolution of intermediates in recombination and DNA repair. Whitby MC, Ryder L, Lloyd RG. Cell. 1993 Oct 22;75(2):341-50.